p53 (Cellular Tumor Antigen p53, Phosphoprotein p53, Tumor Protein p53, Tumor Suppressor p53, TP53, TRP53, Antigen NY-CO-13, FLJ92943, LFS1) (FITC)

Human p53 is a tumor suppressor protein which is mutated in many cancers. p53 expression has been linked to apoptosis caused by DNA-damaging agents and may have a role in cell-mediated cytotoxicity.\n\nApplications:\nSuitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

P1001-26N2

Size

120 tests

Applications

FC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. J.V. Gannon, et al, (1990) EMBO J 9(5): 1595-1602, 2. C.C. Harris and M. Hollstein (1993) New Engl J Med 329: 1318-1327, 3. H. Selter & M. Montenarch (1994) Int J Biochem 26: 145-154, 4. T. Mahdi, et al, (1995) Biol Cell 84: 175-185, 5. M.L. Smith and A.J. Fornace, Jr (1996) Am J Pathol 148: 1019-1022.

Additional Info

Recognizes human P53. Recognizes mutant p53 epitope 156-214 in cells and denatured wild type p53 in Western Blots.\n