Paxillin, phosphorylated (Ser126) (Paxillin alpha, PXN, PXN Protein, FLJ16691, FLJ23042)

Paxillin is a 68kD cytoskeletal adapter protein involved in organization and function of focal adhesions, which are critical to cell adhesion and migration. These cellular events in turn play a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, serine kinases (e.g., FAK, Pyk2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., actin, vinculin, Crk). Serine 126 of paxillin is phosphorylated as a result of Raf stimulation, through the Raf --> MEK --> ERK pathway.\n\nPositive Control: RAW 264.7 cells treated with LPS; 293 cells transfected with wild-type EGFP-tagged human paxillin.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

P3113-91D

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

As reported

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Woodrow, M.A., et al. (2003) Ras-induced serine phosphorylation of the focal adhesion protein paxillin is mediated by the Raf-->MEK-->ERK pathway. Exp. Cell. Res. 287(2):325-338.\nSchaller, M.D. (2001) Paxillin: a focal adhesion-associated adaptor protein. Oncogene 20(44):6459-6472. Review.

Additional Info

Recognizes human Paxillin when phosphorylated at Ser126. Species Crossreactivity: Mouse (100% homologous) paxillin. Chicken (100% homologous) has not been tested, but is expected to react.