Home  >  Products  >  PSME3 (Proteasome Activator Complex Subunit 3, Proteasome Activator 28 Subunit gamma, PA28gamma, PA28g, Activator of Multicatalytic Protease Subunit 3, 11S Regulator Complex Subunit gamma, REG-gamma, Ki Nuclear Autoantigen)
PSME3 (Proteasome Activator Complex Subunit 3, Proteasome Activator 28 Subunit gamma, PA28gamma, PA28g, Activator of Multicatalytic Protease Subunit 3, 11S Regulator Complex Subunit gamma, REG-gamma, Ki Nuclear Autoantigen)

PSME3 (Proteasome Activator Complex Subunit 3, Proteasome Activator 28 Subunit gamma, PA28gamma, PA28g, Activator of Multicatalytic Protease Subunit 3, 11S Regulator Complex Subunit gamma, REG-gamma, Ki Nuclear Autoantigen)

Cat no: P9075-85A

Supplier: United States Biological
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The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome,is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.1-0.3ug/ml, observed in lysates of cell lines Jurkat and K562 on ~30kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: P9075-85A
Reactivities: Human, Mouse, Rat, Canine
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Matsudaira R, Takeuchi K, Takasaki Y, Yano T, Matsushita M, Hashimoto H. Relationships between autoantibody responses to deletion mutants of Ki antigen and clinical manifestations of lupus. J Rheumatol. 2003 Jun;30(6):1208-14.
Additional info: Recognizes human PSME3. Species sequence homology: Canine, mouse and rat.

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