Pyrin Domain-containing Protein 1 (PYDC1, PYC1, ASC2, ASCI, PAAD-only Protein 1, Pyrin-only Protein 1, POP1)

ASC2/POP1 is a PAAD domain only protein originally identified in a bioinformatics screen aimed at understanding molecular apoptosis mechanisms (Pawtokski et al, 2001; reviewed in Fabiola et al, 2006, and Mariathasan and Vuvic, 2003.). ASC2/POP1 has high aa sequence homology with ASC (64%), hence it was originally termed ASC2. The PAAD (also known as PYRIN) domain is a conserved sequence motif identified in more than 35 human proteins with putative functions in apoptosis and inflammatory signaling pathways. PAAD was named after the protein families from which it was discovered: pyrin, AIM (absent-in-melanoma), ASC [apoptosis-associated speck-like protein containing a caspase recruitment domain (CARD], and death-domain (DD)-like. \n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:2000\nImmunohistochemistry (Paraffin): 1:1000-1:5000\nImmunoprecipitation: 1:50-1:200\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSpleen, lymphoid\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9522-75

Size

50ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid, 0.02% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Pawtowski K, F Pio, Z-L Chu, JC Reed and A Godzik 2001. PAAD- a new protein domain associated with apoptosis, cancer and autoimmune diseases. TRENDS in Biochemical Sciences. 26:85-87. 2. Espejo F and ME Patarroyo. 2006. Determining the 3D structure of human ASC2 protein involved in apoptosis and inflammation. Biochemical Biophysical Research Communications. 340:860-864. 3. Mariathasan and Vuvic. 2003. POPing the fire into the pyrin. Biochem J. 373:e1-2.

Additional Info

Recognizes human ASC2. Species Crossreactivity: gerbil, mouse and rat.

Alternative Names

PYD (Pyrin Domain) Containing 1