Rab3A

Rab3A is a 25kD GTP-binding protein implicated in neurotransmitter release (1). The geranylgeranyl groups at the carboxyl-terminus of Rab3A are essential for its association with synaptic vesicles, guanine nucleotide-dissociation factor (GDI), and for GTP binding (2). Rab3A is believed to regulate vesicle fusion through a cycle of GDP/GTP exchange and GTP hydrolysis, which is in turn modulated by GTPase activating protein (GAP), GDI, guanine nucleotide-releasing factor (GRF), and rabphilin (3). The dif-ferent guanine nucleotide binding states of Rab3A is postulated to affect its ability to associate or dissociate with membranes during synaptic vesicle recycling. Its GTP-bound form, which represents the active form of Rab3A, associates with membrane and regulates vesicle docking and fusion. Rab3A and its homolog, Rab3C, have been shown to be co-localized to and dissoci-ated from synaptic vesicles after the stimulation of exocytosis (3). Deletion of the Rab3A structural gene has minimal effect on the morphology of the nervous system and spontaneous and evoked synaptic currents (4). However, Rab3A knockout mice exhibited that an increased rate of synaptic depression after repeated stimulation, suggesting Rab3A is involved in maintaining a steady supply of vesicles for release.\n\nApplications: \nWestern Blot (Colorimetric): 1ug/ml \n\nPositive Controls:\nMouse Brain Tissue Extract

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SPECIFICATIONS

Catalog Number

R0006-75A

Size

25ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in borate buffered saline, pH 8.2, 25mM sodium borate, 100mM boric acid, 75mM sodium chloride, 5mM EDTA disodium salt.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Zahraoui, A. et al. (1989) J. Biol. Chem. 264: 12394-12401. \n2. Guidebook to the Small GTPases. Edited by Marino Zerial and Lukas A. \nHuber. p 312-316 Oxford University Press. \n3. Calakos, N., and Scheller, R. (1996) Physiol. Rev. 76: 1-29. \n4. Geppert, et al. (1994) Nature 369: 493-497. \n

Additional Info

Detects an 25kD protein, corresponding to the apparent molecular mass of Rab3A on SDS-PAGE immunoblots, in samples from mouse and rat. The antibody specificity is confirmed in peptide inhibition immunoblotting studies.