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S100B, Recombinant, Human (Protein S100-B, S-100 Protein beta Chain, S-100 Protein Subunit beta, S100 Calcium-binding Protein B)

Cat no: S0052-18E

S100B, Recombinant, Human (Protein S100-B, S-100 Protein beta Chain, S-100 Protein Subunit beta, S100 Calcium-binding Protein B)

S100 protein family consists of highly homologous Ca2+ binding proteins that possess two EF-hand motifs. Most S100 proteins exist as dimers (frequently homodimers) within cells. Exclusively expressed in vertebrates, S100 is implicated in various intracellular and extracellular regulatory activities. Studies indicate that S100 proteins are involved in the inhibition of protein phosphorylation, inhibition of cytoskeletal constituent assembly, regulation of Ca2+ homeostasis, stimulation of enzyme activities, and interaction with transcription factors. S100B is abundant in the nervous system where it is predominantly expressed in astrocytes, oligodendrocytes and Schwann cells. When secreted by astrocytes, S100B has neurotrophic effects during development and nerve regeneration at physiologic (nanomolar) concentrations. High (micromolar) concentration of S100B, however, has shown to be neurotoxic, participating in the physiology of neurodegenerative disorders.\n\nSource:\nRecombinant corresponding to aa1-92, with N-GST tag, from human S100B, expressed in E.coli.\n\nMolecular Weight:\n~37kD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S0052-18E

Size

500ug

Form

Supplied as a liquid in 20mM HEPES, pH 7.0, 10% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Michetti, F. and Gazzolo, D. (2002) Clinical Chemistry 48:12 2097-2104. 2. Donato, R. (2001) Int J Biochem Cell Biol. 33, 637-668.

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