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SAPK2b, p38 beta, active (Stress-activated Protein Kinase 2b) Recombinant

Cat no: S0096-29

SAPK2b, p38 beta, active (Stress-activated Protein Kinase 2b) Recombinant

Essentially identical proteins in mammals (p38 MAP Kinases) and yeast (HOG-1). Inhibitors include protein phosphatase 2A, PTP-1B, CL-100 and pyridinyl imidazols.\n\nRecombinant, GST fusion-protein corresponding to full length human SAPK2b, expressed in E. coli. MW: ~65kD\n\nSpecific Activity: 55u/ug using MBP as a substrate (1 unit of SAPK2b/p38beta = 1unit of MAPKAP Kinase-2 generated per minute = 1nmol phosphate incorporated into myelin basic protein (MBP) per minute.)\n\nApplications Coupled Kinase Assay: Enzyme was tested in vitro in a coupled kinase phosphorylation assay using 20-100ng of active SAPK2b to activate GST-MAPKAP Kinase-2, unactivated as shown by phosphorylation of MAPKAP Kinase-2 Substrate Peptide. \n\nStorage and Stability: Stable for 6 months at -20 degrees C from date of shipment.

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SPECIFICATIONS

Catalog Number

S0096-29

Size

5ug

Form

5ug recombinant enzyme in 25ul of 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 50% glycerol.

Purity

Purified to > 80% by affinity chromatography on glutathione-Sepharose(TM) beads.

References

1. Cuenda, A., et al., EMBO J. 16: 295

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