Securin (ESP1-associated Protein 1, EAP1, hPTTG, MGC126883, MGC138276, Pituitary Tumor Transforming 1, PTTG, PTTG1, TUTR1)

Securin is a regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Securin acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Securin negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the securin when it is overexpressed (1). It may also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage- response pathways with sister chromatid separation (2). \n \nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50\nWestern Blot: 1:5000-1:20,000\nImmunoprecipitation: 1:20 \nImmunohistochemistry: 1:100-1:250 \nImmunocytochemistry: 1:100-1:250\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

S0379-80A

Size

100ul

Applications

FC, ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-Glycine (pH 7.4), 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195 (2008) 2. Romero F et al. Nucleic Acids Res 29:1300-1307, 2001

Additional Info

Recognizes human securin.