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SEK1, phosphorylated (Ser80) (MKK4, SAPK, Erk Kinase, MKK4, Jun Kinase Kinase, JNKK)

Cat no: J2475-05C

SEK1, phosphorylated (Ser80) (MKK4, SAPK, Erk Kinase, MKK4, Jun Kinase Kinase, JNKK)

SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines (1-3). Activation of SEK1 occurs through phosphorylation of serine and threonine residues at positions 257 and 261, respectively, by MEKK. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at serine 80 inhibits SEK1 and suppresses the stress-activated signal transduction (5).\n\nApplications: \nSuitable for use in Western Blotting, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation 1:25\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

J2475-05C

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

P Type

Pab

Purity

Purified by protein A and peptide affinity chromatography.

Isotype

IgG

References

(1) Davis, R.J. (1994) Trends Biochem. Sci. 19, 470

Additional Info

Detects endogenous levels of SEK1/MKK4 only when phosphorylated at serine 80. Does not crossreact with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3.

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