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SGK, phosphorylated (SGK1, Serum and Glucocorticoid-regulated Protein Kinase)

Cat no: S1010-84

SGK, phosphorylated (SGK1, Serum and Glucocorticoid-regulated Protein Kinase)

Applications: \nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot Analysis: 1:1000-1:3000 detects phospho- SGK1after phosphorylation of unactive SGK1 (S1010-82) with PDK1. Unactive SGK1 was incubated with or without PDK1 for 30 min at 30C. Phosphorylation was termiated by boiling in reducing sample buffer. Aliquots (50ng) were resolved by electrophoresis, transferred to nitrocellulose and probed with S1010-84 (1:3000). Proteins were visualized using a goat anti rabbit secondary antibody cojugated to HRP and a chemiluminescence detection system. Not suitable for use with cell lysates.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (H-DFRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1010-84

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.05% sodium azide, 30% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1.

Additional Info

Recognizes human phospho-SGK, Mr ~48kD. Species sequence homology: rat, mouse and rabbit.

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