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SGK (SGK1, Serum and Glucocorticoid-regulated Protein Kinase (FITC)

Cat no: S1010-83E-FITC

SGK (SGK1, Serum and Glucocorticoid-regulated Protein Kinase (FITC)

The catalytic domain of serum and glucocorticoid-regulated protein kinase (SGK) is 54% identical with that of Akt/PKB. Although SGK1 lacks the PtdIns(3,4,5) P3-binding Pleckstrin-homology domain, it retains the residues that are phosphorylated by PDK1 (Thr256 and Ser422). PDK1 activates SGK in vitro by phosphorylating Thr256. Like Akt/PKB, SGK1 preferentially phosphorylates serine and threonine residues that lie in RXRXXS/T motifs. SGK1 can be activated by PI-3 Kinase, which makes it an important signaling molecule in growth factor and insulin dependent signaling pathways. \n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1.5ug/ml\nELISA: 1ug/ml-3ng/ml\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSp2/0 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1010-83E-FITC

Size

100ul

Applications

ELISA, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to FITC.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human SGK.

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