SGK3, Phosphorylated (Thr320) (Serine/Threonine-protein Kinase Sgk3, Serum/Glucocorticoid-regulated Kinase 3, Serum/Glucocorticoid-regulated Kinase-like, SGK2, SGKL)

Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt. SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways. Induction and activation of SGK has been implicated in activating the modulation of anti-apoptotic and cell cycle regulation. SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK is negatively regulated by ubiquitination and proteasome degradation. SGK3 has been shown to be a downstream signaling molecule in the PI3K pathway. Its activation and phosphorylation at Thr320 by PDK1 may be an Akt-independent manner of signaling in cancer.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1010-85W8

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1.Webster, M.K. et al. (1993) Mol. Cell. Biol. 13, 2031-2040. 2.Kobayashi, T. and Cohen, P. (1999) Biochem. J. 339, 319-328. 3.Park, J. et al. (1999) EMBO J. 18, 3024-3033. 4.Brunet, A. et al. (2001) Mol. Cell. Biol. 21, 952-965. 5.Mikosz, C.A. et al. (2001) J. Biol. Chem. 276, 16649-16654. 6.Hayashi, M. et al. (2001) J. Biol. Chem. 276, 8631-8634. 7.Brickley, D.R. et al. (2002) J. Biol. Chem. 277, 43064-43070. 8.Vasudevan, K.M. et al. (2009) Cancer Cell 16, 21-32.

Additional Info

Recognizes overexpressed levels of human SGK3, phosphorylated at Thr320 at ~62kD