SGLT-1 (Sodium Glucose Transporter 1)

The kidneys play a major role in the regulation of glucose levels. Kidneys filter approx. 180g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, Sodium glucoseco transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes by a facilitated sugar transporter (GLUTs). At least 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species. Individual member of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT1-3 genes code for protein of approx 659-672 residues (calculated size of ~75kD). Both N and C-termini are predicted to be extracellualr. There is approx 60-70% homology between SGLT1-3. SGLTs transport a-methyl-D-glucoside (a-MDG), a non-metabolized model substrate, in Na-dependent manner. SGLT1 does not discriminate a-MDG, glucose, and galactose. SGLT2/3 do not transport D-galactose efficiently.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-10ug/ml using ECL. SGLT-1 is ~70-77kD.\nELISA: 0.5-1ug/ml. Control peptide can be used to coat plates at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nS1010-86C: SGLT-1, Rat Control Peptide\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1010-86G

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Lee WS et al (1994) JBC 269, 12032, Gene accession # AF163846. 2. Hediger, MA et al (1987) Nature 330, 1379-1381. 3. Sileverman , M et al (1993) BBA 1153, 43-52; Pajor, AM et al (1992). 4. Am. J. Physiol. 32, R489-R495; Hirayama, EA et al (1992) BBA 1103, 37-44. 5. Hirayama et al (1991) Am. J. Physiol. 261, C296. 6. Wright EM (2001) Am. J. Renal Physiol. 280, F10

Additional Info

Recognizes rat SGLT-1. No significant sequence homology exists with other SGLTs. Species Sequence Homology: mouse; 93%, human 50%. For human, we recommend S1010-86H.