SH2B1 (PSM, Src-Homology 2 Domain-Containing Protein B1/1B, SH2B1alpha, PSM, SH2B Adaptor Protein 1)

SH2B1 (Src-Homology 2 Domain-Containing protein B1/1B; also SH2B1alpha, PSM and SH2B adaptor protein 1) is a member of the SH2B adapter family of proteins. Although its predicted MW is 80kD, it runs anomalously at 90-102kD in SDS-PAGE. SH2B1 is a nucleocytoplasmic protein that is widely expressed in cells such as skeletal muscle cells, neurons, and adipocytes. It serves as a linker between Jak2 and multiple downstream molecules such as Rac and IRS1. It does this following phosphorylation on a number of potential sites. It also promotes the enzymatic activity of associated receptor kinases, thus potentiating ligand-receptor interactions. SH2B1 appears to bind to nonphosphorylated Jaks as a monomer, while Jak phosphorylation induces SH2B1 dimerization. SH2B1 is further reported to form a homopentamer, and to oligomerize with SH2B2. Human SH2B1(alpha) is 756 amino acids amino acids (aa) in length. It contains one dimerization segment (aa24-85), an NLS (aa224-233), one pleckstrin homology region (aa249-378) and an SH2 domain (aa521-625). At least three utilized phosphorylation sites exist. There are two splice variants for SH2B1. The 90-92kD SH2B1beta isoform shows a 39aa substitution for aa633-756, while the SH2B1gamma isoform possesses a 50aa substitution for the same aa range (i.e., aa633-756). Over aa317-467, human SH2B1 shares 92% aa identity with mouse SH2B1.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts