Shc, phosphorylated (Tyr239/240) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

Shc possesses SH2 and PTB domains. It serves a scaffolding function in signaling for a variety of receptor tyrosine kinases. Shc exists in p46, p52 and p66 isoforms. These isoforms are produced by using alternative translation initiation sites or a differentially spliced message (1-3). In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317 (4-6). Grb2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway (4). Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development (7).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1010-90B

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1. Pelicci, G., et al., Cell 70: 93-104 (1992). 2. Migliaccio, E., et al., EMBO J. 16: 706-716 (1997). 3. Okada, S., et al., J. Biol. Chem. 272: 28042-28049 (1997). 4. Salcini, A.E., et al., Oncogene 9: 2827-2836 (1994). 5. Gotoh, N., et al., EMBO J. 15: 6197-6204 (1996). 6. Van der Geer, P., et al., Curr. Biol. 6: 1435-1444 (1996). 7. Zhang, L., et al., Nat. Immunol. 3: 749-755 (2002).

Additional Info

Detects Shc only when phosphorylated at tyrosine 239/240. May crossreact with activiated EGF receptor protein. Species Crossreactivity: Human, mouse and rat.