SHP 1, Recombinant, Human, GST-Tag (SHPTP-1, SH2 containing protein tyrosine phosphatase 1, PTP1C, SH-PP1, HCP, SHP, PTPN6)

Protein tyrosine phosphatase, full length, (Gene bank accession number NM_080549) with N-terminal GST tag, expressed in an E. coli expression system. \n\nSpecific Activity: \n1U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30 degrees C. \n\nAssay Buffer: \n50mM HEPES, pH 7.4, 2mM EDTA, 3mM DTT, 100mM NaCl, 50mM pNPP. \n\nApplications: \nUseful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

S1013-26H

Size

20ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 75mM NaCl, 0.05% Tween-20, 50% glycerol, 2mM EDTA, 1mM DTT and 10mM glutathione.

Purity

(same/more than) 95% by SDS-PAGE

References

1. N.E. Kilgore et al., J. Immunol. 2003, 4891-4895\n2. J.V. Ravetch & L.L. Lanier Science 2000 290 84\n3. C. Duchesne et al. J. Biol. Chem 2003 14274-14283