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SIGIRR (Single Ig IL-1R-Related Molecule)

Cat no: S1013-68

SIGIRR (Single Ig IL-1R-Related Molecule)

SIGIRR (single Ig IL-1R-related molecule) is a novel member of the interleukin 1 receptor (IL-1R) superfamily (1). Although it shows the typical conserved motifs that characterize the IL-1R and Toll superfamily, it is structurally and functionally distinct from both. SIGIRR has only one Ig domain in its extracellular portion whereas the IL-1R family contains three Ig folds. An unusually long cytoplasmic domain is reminiscent of the structure of drosophila Toll, yet the SIGIRR peptide sequence is more closely related to IL-1RI. A chimera, in which the SIGIRR intracellular domain was fused to the IL-1R extracellular domain, did not activate NFkappaB unlike similar fusion proteins of other IL-1R related molecules. Phylogenetic data that suggest that the plasma membrane protein SIGIRR is a close functional relative of MyD88 in mammals (2). \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000 \nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nRamos whole cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1013-68

Size

200ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.02% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Thomassen E, Renshaw BR, Sims JE. Cytokine 11:389-99 (1999). \n2. Du X, Poltorak A, Wei Y, Beutler B. Eur Cytokine Netw 200011:362-71 (2000).

Additional Info

Recognizes human SIGIRR (Single Ig IL-1R-Related Molecule)

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Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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