SIRT1 (SIRT1 Sir2-like Proteins (Sirtuins) Type 1, Sirtuin, Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. cerevisiae), Sirtuin Type 1, BA57G10.4, hSIRT1, NAD-dependent Deacetylase SIRT1, NAD-dependent Deacetylase Sirtuin-1, SIR2-like

Insulin resistance is an important risk factor in the development of type 2 diabetes and the matabolic syndrome. A new study in Cell Metabolism (1) shows that SIRT1 can ameliorate insulin resistance by silencing expression of protein tyrosine phosphatase 1B.\nSirt1, an HDAC human homologue of the yeast Sir2 protein important in establishing repressive chromatin structures, has been found to associate with the tumor suppressor protein p53. It binds and deacetylates p53 in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress. It has also been shpwn to play a role in increasing lifespan. \n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000-1:10,000\nImmunoprecipitation: 1-4ug/mg lysate\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1013-87T3

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Sun C. et al. SIRT1 Improves Insulin Sensitivity under Insulin-Resistant Conditions by repressing PTP1B. Cell Metabolism 6: 307-319, 2007.

Additional Info

Recognizes human SIRT1. Other species have not been tested. Preliminary data using whole cell lysate from NIH3T3 cells failed to provide evidence that S1013-88A7 will bind mouse Sirt1.