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Smad1, Smad2, Smad3, Smad5 (Mothers Against Decapentaplegic Homolog 1, MAD Homolog 1, Mothers Against DPP Homolog 1, JV4-1, Mad-related Protein 1, SMAD Family Member 1, SMAD 1, Transforming Growth Factor-beta-signaling Protein 1, BSP-1, BSP1, MADH1, MADR1

Cat no: S1014-51V

Smad1, Smad2, Smad3, Smad5 (Mothers Against Decapentaplegic Homolog 1, MAD Homolog 1, Mothers Against DPP Homolog 1, JV4-1, Mad-related Protein 1, SMAD Family Member 1, SMAD 1, Transforming Growth Factor-beta-signaling Protein 1, BSP-1, BSP1, MADH1, MADR1

SMADs are proteins that modulate the activity of transforming growth factor beta ligands. The SMADs,often in complex with other SMADs/CoSMAD, act as transcription factors that regulate the expression of certain genes. Zhu, H et al concluded that targeted ubiquitination of SMADs may serve to control both embryonic development and a wide variety of cellular responses to TGF-beta signals. R-Smads or receptor regulated Smads are a class of proteins that include SMAD1, SMAD2, SMAD3, SMAD5, and SMAD8. In response to signals by the TGF-b superfamily of ligands these proteins associate with receptor kinases and are phosphorylated at an SSXS motif at their extreme C-terminus. These proteins then typically bind to the common mediator Smad or co-SMAD SMAD4.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-51V

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in 5mg BSA, 0.9mg sodium chloride, 0.2mg disodium hydrogen phosphate, 0.05mg thimerosal, 0.05mg sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Smad 1,2,3,5. Species Crossreactivity: Rat.

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