SMAD3 (Mothers Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3, MAD Homolog 3, Mad3, SMAD Family Member 3, SMAD 3, HSPC193, HsT17436, JV15-2, MADH3, MGC60396)

SMAD3, a member of SMAD family of proteins, is involved in TGF-beta signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain.It is a receptor regulated SMAD (R-SMAD) that functions downstream of TGF-beta and activin receptors and mediates their signaling. It is recruited by SARA (Smad anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 disassociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in Childhood T-cell leukemia.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nImmunohistochemistry (formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nLiver\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-53H2

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, Prc

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity Chromatography.

Isotype

IgG

References

1. Qin et.al Genes & Development 16 (15); 1950-1963 (August 1, 2002) 2. Shi et.al Cell 113 (6); 685-700 (13 June, 2003)

Additional Info

Recognizes human SMAD3. Species Crossreactivity: bovine, canine, mouse, porcine, rat, rhesus monkey. Species sequence homology: zebrafish, xenoupus, chicken.

Alternative Names

DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3