SMAD3 (Mothers Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3, MAD Homolog 3, Mad3, SMAD Family Member 3, SMAD 3, HSPC193, HsT17436, JV15-2, MADH3, MGC60396)

Cat no: S1014-53H2

Supplier: United States Biological

0 reviews | Write a Review Pencil

Read More on Supplier Website

Description
Additional Information

SMAD3, a member of SMAD family of proteins, is involved in TGF-beta signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain.It is a receptor regulated SMAD (R-SMAD) that functions downstream of TGF-beta and activin receptors and mediates their signaling. It is recruited by SARA (Smad anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 disassociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in Childhood T-cell leukemia. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Liver Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	S1014-53H2
Reactivities:	Human, Mouse, Rat, Bovine, Canine, Porcine
Hosts:	Rabbit
Applications:	Immunohistochemistry, Western Blot
Size:	100ug
Form:	Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
P type:	Pab
Isotype:	IgG
Purity:	Purified by Protein G affinity Chromatography.
Alternative names:	DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3
References:	1. Qin et.al Genes & Development 16 (15); 1950-1963 (August 1, 2002) 2. Shi et.al Cell 113 (6); 685-700 (13 June, 2003)
Additional info:	Recognizes human SMAD3. Species Crossreactivity: bovine, canine, mouse, porcine, rat, rhesus monkey. Species sequence homology: zebrafish, xenoupus, chicken.

See Full Product Listings >

SMAD3 (Mothers Against Decapentaplegic Homolog 3, Mothers Against DPP Homolog 3, MAD Homolog 3, Mad3, SMAD Family Member 3, SMAD 3, HSPC193, HsT17436, JV15-2, MADH3, MGC60396)

Cat no: S1014-53H2

Get Quote

About Biosave

Product Categories

Information


Get a Quote Direct from United States Biological




By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*