Smad4 (DPC4, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma) (BSA & Azide Free)

DPC4 (also named SMAD4; small mothers against decapentaplegic deleted in pancreatic carcinoma) belongs to the family of SMAD molecules comprising essential mediators of transforming growth factor beta (TGF-b) signalling. To date, seven members of this family have been identified, each of which plays a specific and separate role in mediating TGF-beta superfamily gene transcription. At least two different members, Smad2 and Smad4/DPC4, have been implicated in human cancer and appear to have tumor-suppressor functions whose inactivation may play an important role in the histogenesis of pancreatic and colorectal malignancy and possibly other human cancers as well.\n\nApplications:\nSuitable for use in Immunofluoresence, Immunoprecipitation, and Western Blot. Other applications not tested.\n \nRecommended Dilutions:\nImmunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein G .\nWestern Blot: 1-2ug/ml for 2hrs at RT\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nLS174T, HT29, Raji and Jurkat cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1014-55B

Size

100ul

Applications

IF, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA & Azide. See S1014-55.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Jonson T, Gorunova L, Dawiskiba S, Andren-Sandberg A, Stenman G, ten Dijke P, Johansson B, Hoglund M: Molecular analyses of the 15q and 18q SMAD genes in pancreatic cancer. Genes Chromosomes Cancer 1999;24(1):62-71. 2. No authors listed! SMAD4/DPC4 (small mothers against decapentaplegic deleted in pancreatic carcinoma, locus 4). Tumor suppressor gene. Bull Cancer 1998;85(10):832. 3. Mangray S, King TC: Molecular pathobiology of pancreatic adenocarcinoma. Front Biosci 1998;3:D1148-1160.

Additional Info

Recognizes human SMAD4/DPC4