SMAD6 (SMAD 6, hSMAD6, MADH6, MADH7, HsT17432, MAD homolog 6, SMAD family member 6, Mothers against DPP homolog 6, SMA-AND MAD-related protein 6, SMAD, mothers against DPP homolog 6 (Drosophila), MAD, mothers against decapentaplegic homolog 6 (Drosophila)

Mothers against decapentaplegic homolog 6, also known as SMAD6, is a protein that in humans is encoded by the SMAD6 gene. It belongs to the SMAD family of proteins, which belongs to the TGFb superfamily of modulators. By PCR screening of a somatic cell hybrid panel and YACs, this gene was mapped to 15q22.31. This gene acts as a mediator of TGF-beta and BMP antiflammatory activity. It can suppress 1L 1R-TLR signaling through its direct interaction with PEL1 and prevent NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. SMAD6 may play a role in blocking the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding and binding to regulatory elements in target promoter regions.\n\nUniProt Number:\nO43541\n\nGene ID:\nSMAD6\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144644

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Galvin, K. M., Donovan, M. J., Lynch, C. A., Meyer, R. I., Paul, R. J., Lorenz, J. N., Fairchild-Huntress, V., Dixon, K. L., Dunmore, J. H., Gimbrone, M. A., Jr., Falb, D., Huszar, D. A role for SMAD6 in development and homeostasis of the cardiovascular system. Nature Genet. 24: 171-174, 2000.\n2. Tan, H. L., Glen, E., Topf, A., Hall, D., O'Sullivan, J. J., Sneddon, L., Wren, C., Avery, P., Lewis, R. J., ten Dijke, P., Arthur, H. M., Goodship, J. A., Keavney, B. D.Nonsynonymous variants in the SMAD6 gene predispose to congenital cardiovascular malformation. Hum. Mutat. 33: 720-727, 2012.\n3. Topper, J. N., Cai, J., Qiu, Y., Anderson, K. R., Xu, Y.-Y., Deeds, J. D., Feeley, R., Gimeno, C. J., Woolf, E. A., Tayber, O., Mays, G. G., Sampson, B. A., Schoen, F. J., Gimbrone, M. A., Jr., Falb, D. Vascular MADs: two novel MAD-related genes selectively inducible by flow in human vascular endothelium. Proc. Nat. Acad. Sci. 94: 9314-9319, 1997. \n

Additional Info

Recognizes human h.SMAD6. No crossreactivity with other proteins.