SOCS 1 (Suppressor of Cytokine Signalling 1, Janus kinase (JAK) binding protein, STAT-induced STAT inhibitor, SSI-1)

SOCS (Suppressor of Cytokines Signaling) proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways.\nSOCS 1, also called Janus kinase (JAK) binding protein (JAB), or STAT-induced STAT inhibitor (SSI-1), is an SH2-domain-containing protein. It was shown to interact with all four Jak kinases (Jak1, Jak2, Jak3 and Tyk2) and is induced by and inhibits the signaling action of IL-3, IL-4, IL-6, growth hormone, LIF, prolactin, interferon gamma (IFNg), and erythropoietin (2). It plays an important role in the negative regulation of IFNg signaling and in the differentiation of T cells \n(3). SOCS 1 mRNA is highly expressed in the thymus, to a much lower level in the spleen, and not expressed in most other tissues (4). The SOCS 1 protein is primarily expressed in the thymocytes independent of the cytokine and T cell receptor (5). Biochemical characterization as well as gene disruption studies indicate that SOCS 1 is an important negative regulator of the JAK-STAT signal pathway.\n\nApplications: \nSuitable for use in ELISA (immunogen only), Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-3ug/ml\nImmunoprecipitation: 1-5ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1040-01C

Size

100ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. Zhang, J-G., et al., PNAS 98(23): 13261-13265 (2001). 2. Sporri, B., et al., Blood 97: 221-226 (2001). 3. Krebs, D.L. and Hilton, D.J., J. Cell. Sci. 113: 2813-2819 (2000). 4. Marine, J-C., et al., Cell 98: 609-616 (1999). 5. Blumenstein, M., et al., J. Clin. Endocrinol. Metab. 87(3): 1094-1097 (2002)

Additional Info

Recognizes mouse SOCS 1, NT at ~30kD. Reactivity confirmed with mouse thymus, spleen, lung and liver homogenates as well as with mouse recombinant protein.