SOCS 1 (Suppressor of Cytokine Signalling 1, SOCS1, SOCS-1, Cytokine Inducible SH2 Protein 1, CISH1, Janus Kinase Binding Protein, JAK-binding Protein, JAB, STAT-induced STAT Inhibitor 1, SSI1, SSI-1, TEC-interacting Protein 3, TIP3, TIP-3)

The Suppressor of Cytokine Signaling (SOCS) family of proteins acts in a classic negative feedback loop to regulate cytokine signal transduction. SOCS-1, also designated Janus kinase [JAK] binding protein (JAB), or STAT-induced STAT inhibitor (SSI-1), is a 24kD protein containing an SH2 domain. SOCS-1 inhibits both IL-6-induced receptor phosphorylation and STAT activation. SOCS-1 is also 45 induced by and inhibits the signaling action of IL-3, IL-4, 1 2 3 4kD 97 66 LLIF, prolactin, and erythropoietin. Additionally, SOCS-1 can associate with all 4 JAK family kinases, and is an important regulator of the JAK-STAT signaling pathway. SOCS-1 is found primarily in thymocytes, where it plays a key role in the negative regulation of IFN-g signaling and T cell differentiation.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1ug/ml\nImmunoprecipitation: 10ug/200ul of cell extract from 5x10e6 cells.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSp2/0 with C57BL/6 myeloma cells with spleen cells from Balb/c mice. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1040-01K

Size

100ug

Hosts

Mouse

Reactivities

Mouse

Form

Supplied as a liquid in 100ul PBS, pH 7.2, 50% glycerol.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1) Zhang, J. G., et al. Proc. Natl. Acad. Sci. USA, 98,13261-13265 (2001) 2) Stoiber, D., et al. J. Immunol. 166, 466-472 (2001) 3) Croker, B. A., et al. Nat. Immunol. 4, 540-545 (2003) Clone 4H1 is used in reference number.

Additional Info

Recognizes mouse SOCS-1.