SOCS6, NT (Suppressor of Cytokine Signaling 6, SOCS-6, Cytokine-inducible SH2 Protein 4, CIS-4, Suppressor of Cytokine Signaling 4, SOCS-4, CIS4, SOCS4)

Suppressors of cytokine signaling (SOCS) protein family includes eight members in humans: SOCS-1 through SOCS-7 and cytokine-inducible Src-homology 2 (SH2) containing protein (CIS). All members of the family have similar structure: variable length (50-380aa) and aa sequence N-terminal region, SH2 domain in the center, and homologous C-terminal 40aa long sequence that was named SOCS box. Studies have demonstrated that animals lacking normal SOCS function exhibit high cytokine activity that leads to a variety of inflammatory and autoimmune diseases and even cancer. SOCS-6 is one of the least studied members of the SOCS family. It is 535aa long and has 56% sequence identity with SOCS-7 in region (1-2, 7). Recent studies revealed that SOCS-6 does not interact with Janus kinase family proteins. However, it was found that SOCS-6 binds to KIT membrane region and negatively regulates receptor tyrosine kinases. In addition, studies revealed that SOCS-6 binds to insulin receptor substrate-2, and -4 (IRS-2, and 4) and inhibits downstream signaling.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml\nWestern Blot: 0.5-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1040-06J

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hilton D. J. et al. (1998) Biochemistry 195:114-119. 2. Krebs D. et al. (2001) Stem Cells 19: 378-387. 3. Bullock A. N. et al. (2007) Structure 15(11):1493-1504. 4. Nicholson S. E. et al. (2005) PNAS 102(7):2328-2333. 5. Kario E. et al. (2005) J. Biol. Chem. 280(5):7038-7048. 6. Bayle J. et al. (2004) J. Biol. Chem. 279 (13):12249-12259. 7. Krebs D. L. et al. (2002) Mol. Cel. Biol. 22(13): 4567-4578.

Additional Info

Recognizes human SOCS-6. Species Crossreactivity: mouse.