solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (sodium-potassium-chloride cotransporter isoform 2, BSC1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter, MGC48843, NKCC2, Sol

Supplier:

United States Biological

Cat no: S5329-13

solute carrier family 12 (sodium/potassium/chloride transporters), member 1 (sodium-potassium-chloride cotransporter isoform 2, BSC1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter, MGC48843, NKCC2, Sol

The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na; 1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. SLC12A1 is kidney specific and is essential in the regulation of ionic balance and cell volume. It is a component of an electrically silent transporter system, mediating sodium and chloride reabsorption. Defects in SLC12A1 are the cause of Bartter syndrome type 1, an autosomal recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S5329-13

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Reggiani, L., et al. (2007). Genes Dev. 21: 2358-2370

Additional Info

Recognizes the cytoplasmic domain of human Na+K+Cl- Cotransporter 2. Species Crossreactivity: mouse and rat.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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