Src, phosphorylated (Tyr529) (pp60Src, pp60c-Src)

Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. Tyrosine 529 is located near the carboxyl terminus of Src and acts as a negative regulator, in that Src is held in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus when tyrosine 529 is phosphorylated by Csk. This conformation blocks phosphorylation of the catalytic domain residue (tyrosine 418 in the human sequence), thereby preventing Src activation. When tyrosine 529 is dephosphorylated, tyrosine 418 can be maximally phosphorylated leading to full activation.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilution to be determined by the researcher. \n\nPositive Control: \nChick Embryo Fibroblast (CEF) cells expressing human wild-type Src protein.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S6504-47A

Size

10 Blots

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Ch/Bird

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Recognizes human Src when phosphorylated at Tyr418. Species crossreactivity: mouse, rat, chicken and frog. Species sequence homology: Fyn and Yes (92%).