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SRD5A2 (Steroid 5-alpha-reductase 2, 3 oxo 5 alpha Steroid 4 Dehydrogenase 2, 3-oxo-5-alpha-steroid 4-dehydrogenase 2, 5 alpha-SR2, 5-alpha-SR2, e II 5 alpha Reductase, SR Type 2)

Cat no: S6505-08R

SRD5A2 (Steroid 5-alpha-reductase 2, 3 oxo 5 alpha Steroid 4 Dehydrogenase 2, 3-oxo-5-alpha-steroid 4-dehydrogenase 2, 5 alpha-SR2, 5-alpha-SR2, e II 5 alpha Reductase, SR Type 2)

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA:1:16000\nWestern Blot:1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Testis lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 monthsat -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See S6505-08O-P. Antibody

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SPECIFICATIONS

Catalog Number

S6505-08R

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Moll

Form

Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Torkko KC, van Bokhoven A, Mai P, Beuten J, Balic I, Byers TE, Hokanson JE, Norris JM, BarOn AE, Lucia MS, Thompson IM, Leach RJ VDR and SRD5A2 polymorphisms combine to increase risk for prostate cancer in both non-Hispanic White and Hispanic White men. Clin. Cancer Res. 2008 May 14 (10): 3223-9.

Additional Info

Recognizes human SRD5A2 at ~29kD. Species sequence homology: rat, canine, pig.

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