SREBF2, ID (SREBF2, BHLHD2, SREBP2, Sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2, Sterol regulatory element-binding transcription factor 2, Processed sterol regulatory element-binding protein 2) (PE)

SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. SREBP-2 is ubiquitously detected in various tissues. Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol. LS-B1609 polyclonal antibody detects both precursor and active forms of the protein in tissues and cells such as liver, brown fat, testis, Hep-G2 cells, and human fibroblast. The apparent molecular weight on SDS-PAGE may be higher than the calculated molecular weight (about 126 kDa) due to glycosylation of the protein.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\nImmunohistochemistry: 1:50-100\nImmunofluorescence: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze R-Phycoerythrin conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

042298-PE

Size

200ul

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to R-Phycoerythrin (R-PE).

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Gerin, I., et al. J. Biol. Chem. 285(44):33652-33661(2010)\nBailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)\nMarquart, T.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(27):12228-12232(2010)\nNajafi-Shoushtari, S.H., et al. Science 328(5985):1566-1569(2010)\nPramfalk, C., et al. J. Lipid Res. 51(6):1354-1362(2010)

Additional Info

Human

Alternative Names

SREBF2, BHLHD2, SREBP2, Sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2, Sterol regulatory element-binding transcription factor 2, Processed sterol regulatory element-binding protein 2