SREBP-1 (Sterol Regulatory Element Binding Protein-1, SREBP1, ADD1, D630008H06, Sterol Regulatory Element Binding Transcription Factor 1, SREBF1)

SREBP-1 and-2 are transcription factors which participate in the control of cholesterol homeostasis. SREBP proteins are attached to the endoplasmic reticulum and nuclear envelope. They are proteolytically cleaved and activated in response to conditions of low cellular sterol. Upon activation of SREBP-1 or -2, an ~480-500aa (N-terminal cleavage fragment of these proteins) enters the nucleus, activating transcription of enzymes and other proteins required for cholesterol synthesis. SCA (SREBP-cleavage activity) and caspase-3 proteases cleave SREBPs. SREBP proteins containing point mutations at caspase-3 cleavage sites (Asp460 in SREBP-1 and Asp468 in SREBP-2) do not become cleaved following induction of apoptosis. This suggests that SREBPs may play some role in apoptotic processes.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilutions: \nWestern Blot:1-2ug/ml for 2hrs at RT.\nOptimal dilution determined by the researcher. \n\nPositive Control: \nHeLa and IMR5 cells \n\nCellular Localization: \nCytoplasmic and nuclear \n\nMW of Antigen:\n125kD (precursor)\n60-70kD (cleaved)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S6505-15

Size

250ul

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide. See S6505-15X.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Wang, X., et al., J. Biol. Chem. 270: 15044-18050 (1995). 2. Wang, X., et al., EMBO J. 15: 1012-1020 (1996). 3. Sato, R., et al., J. Biol. Chem. 269: 1726-17273 (1994).

Additional Info

Recognizes human SREBP-1 (Sterol Regulatory Element Binding Protein-1). Species Crossreactivity: hamster.