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STAT 4, phosphorylated (Tyr693) (Signal Transducer and Activator of Transcription 4)

Cat no: S7972-02K

STAT 4, phosphorylated (Tyr693) (Signal Transducer and Activator of Transcription 4)

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. STAT4 is activated when cells are treated with interleukin-12, a key cytokine regulator of cell-mediated immunity. Human STAT4 gene is mapped to chromosome 2q32.2-q32.3.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7972-02K

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Holly A. et al. J. Biol. Chem. 273:17109-17114 (1998). 2. David, F. et al. J. Biol. Chem. 275:2693-2697 (2000).

Additional Info

Recognizes human STAT4, phosphorylated at Tyr693. Species Crossreactivity: mouse and rat.

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