STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5)

Signal Transducer and Activator of Transcription 5 (STAT5, consists of isoforms a and b) is a ~95kD member of a family of transcription factors that are activated to regulate gene transcription in response to multiple hematopoietic cytokines and growth factors. STAT5 is involved in normal cell cycle, survival and apoptosis and is implicated in leukaemogenesis and angiogenesis, as it has been found to be constitutively active in many leukemic cell lines. STAT5 is activated by phosphorylation of tyrosine 694 in STAT5a (tyrosine 699 in STAT5b), a major Src phosphorylation site that is critical for STAT5 dimerization, translocation and binding to DNA. Phosphorylation of tyrosine 694 is also important in mediating erythropoietin-induced signaling and prolactin-induced development and differentiation of mammary epithelial cells.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. \nOptimal dilutions to be determined by the researcher.\n\nPositive Control: 3T3-L1 adipocytes +/- Leukemia Inhibitory Factor (LIF).\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7972-07C

Size

10 Blots

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol. Also available without BSA & Azide. See S7972-07C1.

P Type

Pab

Purity

Purified by sequential epitope-specific chromatography.

Isotype

IgG

References

1. Gewinner, C., et al. (2004) The coactivator of transcription CREB-binding protein interacts preferentially with the glycosylated form of Stat5. J. Biol. Chem. 279(5):3563-3572. 2. Gutierrez-Castellanos, S., et al. (2004) Differences in BCL-X(L) expression and STAT5 phosphorylation in chronic myeloid leukaemia patients. Eur. J. Haematol. 72(4):231-238. 3. Xu, W., et al. (2004) Geldanamycin, a heat shock protein 90-binding agent, disrupts Stat5 activation in IL-2-stimulated cells. J. Cell. Physiol. 198(2):188-196. 4. Rico-Bautista, E., et al. (2004) Downregulation of the growth hormone-induced Janus kinase 2/signal transducer and activator of transcription 5 signaling pathway requires an intact actin cytoskeleton. Exp. Cell. Res. 294(1):269-280. 5. Heath, C. and N.C. Cross (2004) Critical role of STAT5 activation in transformation mediated by ZNF198-FGFR1. J. Biol. Chem. 279(8):6666-6673. 6. Shipley, J.M. and D.J. Waxman (2003) Down-regulation of STAT5b transcriptional activity by ligand-activated peroxisome proliferator-activated receptor (PPAR) alpha and PPARgamma. Mol. Pharmacol. 64(2):355-364. 7. Okutani, Y., et al. (2001) Src directly tyrosine-phosphorylates STAT5 on its activation site and is involved in erythropoietin-induced signaling pathway. Oncogene 20(45):6643-6650.8. Gesbert, F. and J.D. Griffin (2000) Bcr/Abl activates transcription of the Bcl-X gene through STAT5. Blood 96(6):2269-2276.

Additional Info

Recognizes human STAT5 phosphorylated at Tyr694. Species Sequence Homology: mouse STAT5; human and rat STAT5a: 100%; human STAT5b: 100%; mouse and rat STAT5b: 92%.