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STAT 5b (Signal Transducer and Activator of Transcription 5b)

Cat no: S7972-15F

STAT 5b (Signal Transducer and Activator of Transcription 5b)

Applications:\nWestern Blotting: 0.5-2mg/ml detects STAT5B (doublet of 90kD and 92kD) in\nhuman A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure.\n\nImmunoprecipitation: 4ug immunoprecipitates STAT5B from A431 carcinoma cells and a\nprevious lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500ug protein) was incubated with 4ug of anti-STAT 5B Immunecomplexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection.\n\nPositive Antigen Control: mouse 3T3 cell lysate. Add 2.5ul of 2-mercaptoethanol\nper 100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7972-15F

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Suppied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Lin et al., J. Biol. Chem. 271:10738, 1996

Additional Info

Recognizes the 90-92kD forms of STAT5B protein.Species Crossreactivity: Human and mouse.

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