STAT3, Phosphorylated (Tyr68) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)

This protein is a member of the family of STAT proteins. These proteins are latent cytoplasmic transcription factors with the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. The Stat3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, onM, LIF, CNTF, G-CSF, IL-12, IL-2, GH, EGF, PDGF, and CSF-1. Stat3 is not activated by IFN beta. One active Stat3 translocates to the nucleus and binds to DNA as either a homodimer or as a heterodimer with Stat1. Transcription factors associated with Stat3 include cJun and cyclic AMP responsive enhancer binding protein (CREB). Stat3 interacts with the APRE (acute phase response element) and the SIE (c-sis inducible element).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nPMA treated and untreated MOLT4 cell lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029618

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Kisseleva, T., et al. (2002). Gene. 285:1-24. 2. Heim, M. (1999). J. Recept. Signal Transduct. Res. 19:75-120. 3. Bromberg, J., et al. (1999). Cell. 98: 295-303. 4. Wen, Z., et al. (1995). Cell. 82:241-250.

Additional Info

Recognizes human STAT3 phosphorylated at Tyr68. Species sequence homology: Mouse, rat, bovine, porcine, xenopus, chicken (100%).