STAT3, phosphorylated (Tyr705) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF) (Alexa Fluor(R) 647)

Stat3 is a key signaling molecule for many cytokines and growth-factor receptors (1) and is required for murine fetal development (2). Additionally, Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 via the MAPK or mTOR pathway (8,9). Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3a (86kD) and Stat3b (79kD) depend on cell type, ligand exposure or maturation stage of the cells (10). It is notable that Stat3b lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).\n \nNotes on Use: Add 10 microl of the conjugated antibody to 500,000 cells in 90 microl PBS/0.5% BSA. See protocol for more details. \n\nRecommended Dilution: \nFlow Cytometry 1:10 \nOptimal dilutions to be determined by the researcher.\n\nStorage: \nStore at 4 degrees C. Protect from light. Do not freeze.

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SPECIFICATIONS

Catalog Number

S7971-01E2

Size

500ul

Applications

ELISA, FC

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2, less than 0.1% sodium azide, 2 mg/ml BSA. Conjugated to Alexa Fluor(R) 647 under optimal conditions with an F/P ratio of 2-5.

P Type

Mab

Purity

Purified prior to conjugation.

Isotype

IgG

References

Heim, M.H. (1999) J. Recept. Signal Transduct. Res. 19, 75-120.\nTakeda, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 3801-3804.\nCatlett-Falcone, R. et al. (1999) Immunity 10, 105-115.\nGarcia, R. and Jove, R. (1998) J. Biomed. Sci. 5, 79-85.\nBromberg, J.F. et al. (1999) Cell 98, 295-303.\nDarnell Jr., J.E. et al. (1994) Science 264, 1415-1421.\nIhle, J.N. (1995) Nature 377, 591-594.\nWen, Z. et al. (1995) Cell 82, 241-250.\nYokogami, K. et al. (2000) Curr. Biol. 10, 47-50.\nBiethahn, S. et al. (1999) Exp. Hematol. 27, 885-894.

Additional Info

Detects endogenous levels of Stat3 only when phosphorylated at Tyr705. Does not crossreact with phospho-EGFR or with corresponding phospho-tyrosines of other Stat proteins. Species Crossreactivity: Mouse.