Human STAMP2, also called Steap4 or TIARP (TNF-alpha-induced adipose-related protein), is a 55kD, 459aa, 6-transmembrane (TM) member of the Steap family of metalloreductases. It is induced by androgens in androgen-regulated prostate cells, and is upregulated during adipocyte differentiation by TNF-alpha. Full-length human and mouse STAMP2 share 80% aa identity. A 283aa variant lacks a portion of the oxidoreductase domain and three TM domains. STAMP2 is present in the Golgi complex and early endosomes in addition to the cell surface.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions:
Flow Cytometry: 25ug/ml; Use 10ul to label 1-2.5x10e5 cells in total reaction volume (same/less than)200ul.
Optimal dilutions to be determined by the researcher.
Positive Control:
A549 cells
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.