Stella (Development Pluripotency-associated 3, DPPA3, Primordial Germ Cell 7, PGC7)

Stella was initially identified in primordial germ cells and pre-implantation embryos whose expression as a maternal factor is important in early embryonic development but is not required for germ cell specification in mice. In humans, Stella is thought to be a marker for pluripotency in embryonic stem (ES) cells as its expression is observed in primordial germ cells of both sexes and germ cell tumors but not in normal somatic tissues. However, in ES cell colonies, heterogeneous expression of Stella was seen in high throughput in situ hybridization assays, indicating that higher levels of complexity exist in otherwise thought to be undifferentiated ES cells. At least two distinct isoforms of Stella are known to exist.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: 293 Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7972-90

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

PBS containing 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Sato M, Kimura T, Kurokawa K, et al. Identification of PGC7, a new gene expressed specifically in preimplatation embryos and germ cells. Mech. Dev. 2002; 113:91-4. 2. Bortvin A, Goodheart M, Liao M, et al. Dppa3/Pgc7/stella is a maternal factor and is not required for germ cell specification in mice. BMC Dev. Biol. 2004; 4:2. 3. Bowles J, Teasdale RP, James K, et al. Dppa3 is a marker of pluripotency and has a human homologue that is expressed in germ cell tumours. Cytogenet. Genome Res. 2003; 101:261-5.\n4. Carter MG, Stagg CA, Falco G, et al. An in situ hybridization-based screen for heterogeneously expressed genes in mouse ES cells. Gene Expr. Patterns 2008; 8:181-8.

Additional Info

Recognizes human Stella. Species Crossreactivity: Mouse and rat.