Structural Maintenance of Chromosomes 1, phosphorylated (Ser957) (SMC1, bK268H5, bK268H5.5, CDLS2, DKFZp686L19178, DXhXs423e, DXS423E, FLJ43748, KIAA0178, MGC138332, OTTHUMP00000061876, RP6-29D12.1, Sb1.8, SB1.8/DXS423E Protein, Segregation of Mitotic Chr

Involved in chromosome cohesion during cell cycle and in DNA repair and is a central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. SMC1 is involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, and works as a downstream effector in the ATM/NBS1 branch of S-phase checkpoint.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S7980-01E1

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Tris-citrate/phosphate [pH7-8]

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Lukas, C., et al. Distinct spatiotemporal dynamics of mammalian checkpoint regulators iduced by DNA damage. Nature Cell Biology. 5:255-260, 2003.\n2. Musio, et al. Hum Mol. Genet. 14(4): 525-533, 2005.\n3. Ismail, et al. Activation of ataxia telangiectasia mutated by DNA strand break-inducing agents correlates closely with the number of DNA double strand breaks. J. Biol. Chem. 280(6): 4649-4655, 2005. \n4. Goldberg, et al. Nature. 421(6926): 952-956, 2003. \n5. Mochan, et al. Cancer Res. 63(24): 8586-8591, 2003.\n6. Yazdi, et al. Genes Dev. 16(5): 571-582, 2002.

Additional Info

Reacts with human SMC1 phosphoSerine 957 Species Crossreactivity: reacts with human SMC1 phosphoSerine 957. Other species have not been tested.