Superoxide Dismutase, Mn (SOD Mn)

Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide (1, 2, 3). There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (CuZn SOD) and manganese-containing SOD (Mn SOD, SOD-2). The CuZn form contains Cu and Zn ions and exists as a 32kD dimer in the cytosol. Mn SOD is an 80kD tetramer that contains Mn ion and resides in the mitochondrial matrix. Mn SOD is a tumor necrosis factor (TNF)-inducible enzyme that protects cells from TNF-mediated apoptosis (4) via superoxide anion detoxification and the subsequent regulation of apoptosis through cytochrome c release and the modulation of the redox state of the mitochondria (6). Mn SOD has also been shown to be a tumor suppressor in human breast cancer (7). Overexpression of this enzyme protects neurons from NMDA-and nitric oxide-induced neurotoxicity (5).\n \nApplications:\nSuitable for use in Western Blot and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot (Colorimetric) (8): 0.5ug/ml \nFlow Cytometry: 10ug/ml.\nOptimal dilutions to be determined by researcher.\n \nPositive Control:\nRat Brain Tissue Extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S8060-10B

Size

25ug

Applications

FC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, Dro/Arth, GP, NHP, Prc, Rabbit, Sheep, Xen/Amph

Form

Supplied as a liquid in PBS, pH 7.0, 15mg/ml BSA, 0.1% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Kurobe, N., Inagaki, T., Kato, K. (1990) Clinica Chimica Acta 192: 171-180. 2. Kurobe, N., Suzuki, F., Okajima, K., Kato, K. (1990) Clinica Chimica Acta 187: 11-20. 3. Wong, G.H., Elwell, J.H., Oberley, L.W., Goeddel, D.V. (1989) Cell 58: 923-931. 4. Manna, S.K., Zhang, H.J., Yan, T., Oberley, L.W., Aggarwal, B.B. (1998) J. Biol. Chem. 273: 13245-13254. 5. Gonzalez-Zulueta, M., Ensz, L.M., Mukhina, G., Lebovitz, R.M., Zwacka, R.M., Engelhardt, J.F., Oberley, L.W., Dawson, V.L., Dawson, T.M. (1998) J. Neurosci 18: 2040-2055. 6. Li, Y., Huang, T.T., Carlson, E.J., Melov, S., Ursell, P.C., Olson, J.L., Noble, L.J., Yoshimura, M.P., Berger, C., Chan, P.H. (1995) Nat. Genet. 11: 376-381. 7. Ojika T., Imaizumi,M., Watanabe, H., Sakakibara, M., Abe, T., Kato, K. (1991) Acta Histochem Cytochem 24(5): 489-495. 8. Kurobe, N., Kato, K. (1991) Biomed. Rsch. 12: 97-103. 9. Xiao-Hong Liu, et al., (1993) Brain Res. 625: 29-37. 10. Bidmon, H-J., Kato, K., Schleicher, A., Witte, O.W., Zilles, K. (1998) Stroke 29: 203-211. 11. Hadjur, S., Ung, K., Wadsworth, L., Dimmick, J., Rajcan-Separovic, E., Scott, R.W., Buchwald, M., Jirik, F.R. (2001) Blood 98(4): 1003-1011. 12. Katsuki, H., Tomita, M., Takenaka, C., Shirakawa, H., Shimazu, S., Ibi, M., Kume, T., Kaneko, S., Akaike, A. (2001) J Neurochem 76(5): 1336-1345.

Additional Info

Recognizes a rat ~25kD protein, corresponding to the apparent molecular mass of rat Mn superoxide dismutase (SOD). Species Crossreactivity: human, mouse, bovine, canine, chicken, Drosophila, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus.