Surfactant Protein B (SP-B)

There are four surfactant-specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant. SP-B is synthesized by the alveolar type II epithelial cells as a 40-42 kD precursor that is subsequently proteolytically \nprocessed to 7.8-8 kD. SP-B enhances the spreading and stability of surfactant phospholipids in the alveolus. SP-B is essential for air-breathing in mammals and is therefore largely conserved. SP-B can interact with both phospholipid head groups and fatty chains and is particularly active in enhancing surface active behavior in endogenous and exogenous lung surfactants. Even low SP-B contents had measurable effects in increasing the adsorption, dynamic surface tension lowering, and/or film respreading of DPPC, mixed synthetic lipids, and column-purified lung \nsurfactant phospholipids. Deficiency of SP-B and other surfactant components is associated with respiratory distress syndrome (RDS) in premature infants and adults with respiratory distress syndrome (ARDS). \n\nApplications:\nSuitable for use in Immunoassays and Western Blot.\nOther applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S8401-02C

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1. Oviedo, J et al; Quantitation of pulmonary surfactant protein SP-B in the absence or presence of phospholipids by enzyme-linked immunosorbent assay. Anal Biochem 2001, 293: 78 2. Perez-Gil, J et al; Lipid-protein interactions of hydrophobic proteins SP-B and SP-C in lung surfactant assembly and dynamics. Pediatr Pathol Mol Med 2001, 20: 445

Additional Info

Recognizes the human surfactant protein B (SP-B). The antibody rescognizes under nonreducing conditions the most prominent forms of SP-B of 8kD and 22kD, corresponding to SP-B dimers. \nThe polyclonal antibody is cross reactive with human SP-B.