SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1, Histone H3-K9 Methyltransferase 1, H3-K9-HMTase 1, Su(var)3-9 Homolog 1, KMT1A, Histone-lysine N-methyltransferase SUV39H1, Position-effect Variegation 3-9 Homolog, Lysine N-methyltransferase 1A)

Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger geneRIZ1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ1 is also a tumor susceptibility gene in mice. The loss of RIZ1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ1 promoter strongly correlated with lost or decreased RIZ1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S8950-01B

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Fujita, N., et al., J. Biol. Chem. 278(26):24132-24138 (2003). 2. Macaluso, M., et al., Oncogene 22(23):3511-3517 (2003). 3. Schotta, G., et al., EMBO J. 21(5):1121-1131 (2002). 4. Vaute, O., et al., Nucleic Acids Res. 30(2):475-481 (2002). 5. Rea, S., et al., Nature 406(6796):593-599 (2000).

Additional Info

Recognizes human SUV39H1. Species Crossreactivity: mouse.