Syk, p72

Syk plays an important role in mast cell response, initiated by the aggregation of the IgE high affinity receptor Fc epsilon R1. Phosphorylation of ITAMs within Fc receptor R1 by another tyrosine kinase Lyn, results in the recruitment of Syk, which is then responsible for the further phosphorylation of numerous target proteins within many different signal transduction pathwaysFurther studies have shown that Syk also plays a major role in B cell receptor-mediated apoptosis, proliferation and haematopoiesis. Syk is also reported to associate with protein kinase C alpha during platelet activation.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitatio . Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: detects a band of approximately 72kD in Rames & RBL cell lysates.\nOptimal dilution determined by the researcher. \n\nRecommended Secondary Reagents: \nI1904-65W: IgG, F(ab

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SPECIFICATIONS

Catalog Number

S9100-02B

Size

100ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

P Type

Mab

Purity

Purified by protein A affinity chromatography.

Isotype

IgG1

References

1. Amoui, M. et al. (1997). Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type I Fc epsilon receptors. Eur. J. Immunol. 27(1): 321-328. 2. Tolar, P. et al. (1997). Protein tyrosine kinase Syk is involved in Thy-1 signaling in rat basophilic leukemia cells. Eur. J. Immunol. 27(12): 3389-3397. 3. Manetz, T.S. et al. (2001). Vav1 regulates phospholipase C gamma activation and calcium responses in mast cells. Mol. Cell. Biol. 21(11): 3763-3774. 4. Swann, P.G.et al. (1999). Requirement for a negative charge at Threonine 60 of the FcR gamma for complete activation of Syk. J. Biol. Chem. 274(33): 23068-23077. 5. Arudchandran, R.et al. (2000). The src homology 2 domain of Vav is required for its compartmentation to the plasma membrane and activation of c-Jun NH2-terminal kinase 1. J. Exp. Med. 191(1): 47-59.

Additional Info

Recognizes protein tyrosine kinase p72syk (Syk), a 72kD non-receptor tyrosine kinase, critical for immunoreceptor signaling in haematopoietic cells, but also expressed by nonhaematopoietic cells, including fibroblasts, endothelial cells, and hepatocytes.\nSpecies Cross Reactivity: Human, mouse and rat.