Synaptotagmin-6 (P65,SVP65, DKFZp781D2042, SYT)

Synaptotagmins constitute a family of membrane trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including\ntransmembrane (Ferlins, E-Syts, and MCTPs) and soluble RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.\nCalcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2.\n\nSynaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated\nprotein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2 (1, 2).\n\nSynaptotagmin-6 is localized to the endoplasmic reticulum and/or Golgi-like perinuclear compartment. It may be important for trafficking and calcium signaling as\nit is specially expressed in non-neuronal tissues. Also SytVI is present in the acrosomal region of mammalian spermatozoa. The cytosolic domain of SytVI can abrogate exocytosis by competing with the endogenous protein for essential interactions with the fusion machinery involved\nin a acrosomal exocytosis (3-5).\n\nApplications: \nWestern Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\n1ug/ml was sufficient for detection of Synaptotagmin-6 in 20ug of rat brain lysate by Western Blot using Goat anti-mouse IgG:HRP as the secondary antibody.\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167610

Size

100ug

Applications

ICC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS pH7.4, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Schengrund C.L., et al. (2002) J Biol Chem. 277: 32815.\n2. Reichardt L.F., et al. (1981) J Cell Biol. 91:257.\n3. Roggero C.M., et al. (2006) Dev Biol. 285(2): 422-435.\nButz S., Fernandex-Chacon R., Schmitz F., Jahn R.\nSudhof T.D., (1999) J Biol Chem. 274(26): 18290-\n18296.\nMikoshiba K., Fukuda M., Ibata K., Kabayama H.,\nMizutani A. (1999) Chem Phys Lipids. 98(1-2): 59-67.\n

Additional Info

Recognizes mouse Synaptotagmin-6. Species Crossreactivity: Rat, Mouse, Human. Detects ~55kD. Does not cross-react with other synaptotagmins