Synaptotagmin, phosphorylated (Ser309) (SYT, Synaptotagmin-1, SYT1, Synaptotagmin I, SytI, p65, SVP65)

Neurotransmitter release is mediated by exocytosis of synaptic vesicles at the presynaptic active zone of nerve terminals (1). Synaptic vesicles undergo a trafficking cycle to support rapid and repeated cycles of neurotransmitter release. Calcium-triggered exocytosis is the focal point of the vesicle cycle, which is followed by different routes of endocytosis and recycling. Recycling then leads to the docking and priming of the vesicles for another round of exocytocis and endocytosis. The synaptotagmins are integral membrane proteins of synaptic vesicles. Synaptotagmins may serve as the calcium sensors that trigger neuortransmitter release (2,3). More than 13 synaptotagmins have been described, and synaptotagmin I is among the best characterized isoforms. Synaptotagmin I is an abundant constituent of synaptic vesicles that binds calcium ions through two C2 domains. It is thought that synaptotagmin I triggers exocytosis in a calcium dependent manner, and may also play a key role in endocytosis following membrane fusion. Synaptotagmin is phosphorylated by multiple protein kinases, and these phosphorylation events may play a regulatatory role in both exocytotic and endocytotic events.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRat brain\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S9108-99P

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM HEPES (pH 7.5), 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Sudhof TC. 2004. The synaptic vesicle cycle. Annu Rev Neurosci. 27:509-47. 2. Bai J and Chapman ER. 2004. The C2 domains of synaptotagmin--partners in exocytosis. Trends Biochem Sci. 3:143-51.\n3. Chapman, E. 2002. Synaptotagmin: A Ca2+ sensor that triggers exocytosis. Nature Rev Mol Cell Biol. 3:498-508.

Additional Info

Recognizes human Synaptotagmin, phosphorylated at (Ser309). Species crossreactivity: mouse and rat.

Alternative Names

DKFZp781D2042