Synuclein, alpha (SNCA, 127750, P37840, MGC110988, NACP, PARK1, PARK4, non-A beta Component of AD Amyloid, NACP, Parkinson Disease Familial 1, PD1)

alpha-Synuclein is a small (140 amino acid) cytoplasmic protein of unclear function that is enriched in presynaptic terminals and is the precursor protein of a nonamyloid beta component of senile plaques in Alzeheimer's Disease (AD). Alpha-Synuclein is located predominantly in the presynaptic nerve terminals in the brain but is also found in low concentrations in all tissues except liver. Recent studies have shown that abnormal aggregation and accumulation of synaptic proteins, including alpha-Synuclein might be associated with plaque formation in AD and seems to be a major component of Lewy bodies in dementia with Lewy bodies (DLB). It has also been identified in the Glial Cytoplasmic Inclusions (GCIs) found in Multiple System Atrophy (MSA).\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 shows a band at 14-19kD\nImmunohistochemistry (frozen or paraffin sections): \nFrozen sections: 1:4000-1:12,000\nParaffin sections: 1:1000-1:2000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHuman brain sections\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, ddh2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S9500-04

Size

50ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with 50ul sterile 40-50% glycerol, ddH2O.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Iwai, A, et al., Neuron (1995) 14:467

Additional Info

Recognizes human synuclein, alpha. Immunohistochemistry and \nWestern Blot analysis of human brain indicates a high level of\nspecificity. Crossreactivity with beta-synuclein is minimal. \nSpecies Crossreactivity: rat.