TACE (Disintegrin and Metalloproteinase Domain-containing Protein 17, TNF-alpha-converting Enzyme, TNF-alpha Convertase, Snake Venom-like Protease, CD156b Antigen, CSVP, TNF-a Converting Enzyme, ADAM17)

b-amyloid (Ab) deposition in the brain is the hallmark of Alzheimer's Disease (AD). To initiate Ab formation, b-secretase cleaves APP at the N-terminus of Ab to release APPsb (~100kD soluble NT-fragment), and C99, a 12kD CT membrane fragment. Alternatively, a-secretase cleaves within the Ab to prevent the formation of Ab. Cleavage by a-secretase produces a soluble Nterminal fragment, APPsa, and a 10kD membrane C-terminal fragment, C83. Both C99 and C83 can be further cleaved by gsecretase releasing Ab and a nonpathogenic p3 peptide, respectively. Recently TACE, a member of the ADAM family (A Disintegrin And Metalloprotease family) protease has been shown to play a central role in a regulated cleavage of human APP. Inhibition of TACE affects both APP secretion and Ab formation in cultured cells. Membrane-bound TNF-a, like APP, is transmembrane protein that can undergo TACE-mediated proteolysis to release the extracellular domain as soluble TNF-a. TACE contain an autoinhibitory domain that must be removed for activity, a proteolytic domain, a disintegrin domain, a cysteine-rich domain, and a Transmembrane domain.\n\nApplications:\nSuitable for use in Western Blot, ELISA and Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-10ug/ml (ECL). The major form of recombinant TACE migrates as 70kD.\nELISA: 1:10,000-1:100,000\nFlow Cytometry: 500-1ug/10e6 cells (blood lymphocytes or THP-1 cells).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T0500-01H

Size

100ug

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute in 100ul sterile ddH2O.

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Buxbaum JD et al (1998) J. Biol. Chem. 273, 27765-27767. 2. Hall L (1998) Gene Accession # AJ012603. 3. Lammich S et al (1999) PNAS 96, 3922-3927. 4. Vassar R et al (1999) Science 286, 735-741. 5. Yan R et al (1999) Nature 402, 533-537. 6. Sinha S et al (1999) Nature 537- 540. 7. Hussain I et al (1999) Mol. Cell Neurosci. 14, 419-427. 8. Lin X et al (2000) PNAS 97, 1456-1460. 9. Black et al (1997) Nature 385, 729. 10. Moss et al (1997) Nature 385, 733.

Additional Info

Recognizes human TACE. Minimal reactivity with bace, ADAM8, -9, -10, and -15.