TAF10, ID (TAF10, TAF2A, TAF2H, TAFII30, Transcription initiation factor TFIID subunit 10, STAF28, Transcription initiation factor TFIID 30kD subunit) (Biotin)

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

042574-Biotin

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Egloff, S., et al. J. Biol. Chem. 285(27):20564-20569(2010)\nMa, Y., et al. J. Biol. Chem. 285(13):9813-9822(2010)\nZhao, Y., et al. Mol. Cell 29(1):92-101(2008)\nHao, H., et al. Oncogene 26(57):7872-7884(2007)\nCouture, J.F., et al. Nat. Struct. Mol. Biol. 13(2):140-146(2006)

Additional Info

Human

Alternative Names

TAF10, TAF2A, TAF2H, TAFII30, Transcription initiation factor TFIID subunit 10, STAF28, Transcription initiation factor TFIID 30kD subunit