Talin (KIAA1027, TLN, TLN1, ILWEQ)

Immunoblots the 225kD talin band on SDS gels of platelets and other cells. It may be used to stain adhesion plaques in fixed and permeabilised fibroblasts. T0750-20A recognizes an epitope within residues 139-433. This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.\n \nApplications:\nSuitable for use in Flow Cytometry, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 1:25-1:100; 10ul labels 10e6 cells in 100ul. Membrane permeabilisation is required.\nImmunohistochemistry: Frozen sections\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSpleen cells from immunized BALB/c mice were fused with cells of the SP2/0 mouse myeloma cell line. \n\nPositive Control: \nMost tissues \n\nRecommended Negative Control:\nI1904-78R: IgG1 Murine Negative Control\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap

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SPECIFICATIONS

Catalog Number

T0750-20A

Size

200ug

Applications

FC, IHC, IP

Hosts

Mouse

Reactivities

Hum, Ch/Bird, Rabbit

Form

Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

11. Porter, R.M. et al. (1993) Monoclonal antibodies to cytoskeletal proteins: An immunohistochemical investigation of Human Colon Cancer. J. Pathol. 170: 435-440. 2. Bolton, S.J. et al. (1997) Monoclonal antibodies recognising the N

Additional Info

Recognizes an epitope within residues 139-433 of human talin at ~225kD. Stains adhesion plaques in fixed and permeabilized fibroblasts. Species Crossreactivity: rabbit, chicken