Tankyrase 2 (TNKS2, Tankyrase, TRF1-interacting Ankyrin-related ADP-ribose polymerase 2, PARP-5b, PARP-5c, PARP5B, PARP5C, TANK2, TNKL, Tankyrase 2)

Tankyrase (TRF1-interacting ankyrin-related ADP-ribose polymerase, Tankyase 1, TANK1, TNKS1) is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP) (1). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. TRF1 is a negative regulator of telomere length maintenance. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. During interphase tankyrase co-localizes with TRF1 to telomeres plus nuclear pore complexes whereas during mitosis tankyrase localizes around the pericentriolar matrix of mitotic chromosomes. Tankyrase 2 (TRF1-interacting akyrin-related ADP-ribose polymerase 2, TANK2, TNKS2) is a closely related homolog of tankyrase 1. Tankyrase 2 lacks the N-terminus HPS domain (containing homo-polymeric runs of histidine, proline, and serine) found in tankyrase 1. \n\nApplications: \nSuitable for use in Peptide ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nPeptide ELISA: 1:16,000\nWestern Blot: 0.5-1.5ug/ml. Observed band at ~125kD. An additional band of unknown identity was observed at ~45kD.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nJurkat cell line lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T0900-09A

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

References

1. Sidorova N, Zavalishina L, Kurchashova S, Korsakova N, Nazhimov V, Frank G, Kuimov A. Immunohistochemical detection of tankyrase 2 in human breast tumors and normal renal tissue. Cell Tissue Res. 2006 Jan;323(1):137-45. Epub 2005 Sep 3. PMID: 16151859

Additional Info

Recognizes human Tankyrase 2 at ~125kD. Species Sequence Homology: mouse and canine