Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase, TANK2, TNKL)

The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. Introduction of telomerase into normal human cells has been shown to extend normal cell life by ~ 20 doubling. Poly(ADP-ribose) polymerases (PARPs) catalyze formation of long, branched chain of poly(ADP-ribose) onto protein acceptors using NAD+ as a substrate. Poly(ADP)ribosylation is a transient posttranslational modification that can either enhance or reduce protein activity. Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase; human 1327 aa, renamed as TNKS-1/TANK1, chromosome 8), a modular protein with homology to ankyrin and poly(adenosine diphosphate-ribose) polymerase (PARP) has been cloned and localized to telomere. The N-terminal HPS domain contains multiple run of histidine, proline, and serine residue homopolymers. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml. Using ECL. TANK1 ~130kD.\nELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nT0900-08: Tankyrase, Human (TRF1 interacting ankyrin-related ADP-ribose polymerase, TANK2, TNKL)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T0900-25

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kuimov AN et al (2001) Genes Immuno. 2, 52; 2. Lyons RJ et al (2001) JBC 276, 17172; 3. Kaminker PG et al (2001) JBC 276, 25891; 4. Sboido JI et al (2002) Biochem. J. 361, 451; 5. hi-N-W et al (200) JBC 275, 38347.

Additional Info

Recognizes human Tankyrase (TRF1 interacting ankyrin-related ADP-ribose polymerase, TANK2, TNKL). Species sequence homology: 92%, rat; 85%, mouse.